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QIAGEN QIAcuity
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QIAGEN QIAcuity fully integrated solutions feature an automated workflow with the more conventional pipetting of reaction mixtures into 96 or 24 well plates (dedicated QIAGEN digital PCR nanoplates).

SKU
QIAcuity One, 2plex System
Model

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QIAGEN QIAcuity fully integrated solutions feature an automated workflow, combined with faster analysis times, scalability, higher multiplexing and higher throughput than other digital PCR platforms on the market. QIAcuity systems offer a greatly simplified emulsion-free workflow, with the more conventional pipetting of reaction mixtures into 96 or 24 well plates (dedicated QIAGEN digital PCR nanoplates).

Results The QIAcuity Digital PCR System is designed to provide accurate quantitative results for mutation detection, copy number variation (CNV), gene expression studies, gene editing analysis, and more. This nanoplate-based system can fully automate standard dPCR workflows for sample distribution, PCR amplification, and imaging in a fraction of the time.

QIAGEN QIAcuity is a Nanoplate-Based Digital PCR System

The four QIAcuity digital PCR systems of different models on the market have the highest 5-color fluorescence detection channel and a 1-channel reference fluorescence channel, which can effectively monitor the loading conditions in the nanochip holes and obtain more accurate results. Not only that, QIAcuity Eight can also accommodate the throughput of 8 chips, and the internally loaded dual thermal cycle module achieves a throughput of up to 1,248 samples in 8 hours.

  1. Integrated digital PCR system
  2. Product variations according to the required number of treatments (1, 4 and 8 plate treatment)
  3. Multiplex performance that goes one step further (up to 5 plexes)
  4. Flexible sample throughput
  5. Only 2 hours to result

QIAGEN QIAcuity is a digital PCR that uses the Nanoplate-Based Partition principle to measure gene expression. Which is considered to be the 3rd Generation of the current Thermal Cycler family. The machine has a working principle by dividing small proportions of PCR Reaction (Partitioning) on ​​the Nanoplate, then increasing the amount of genetic material by Polymerase Chain Reaction (PCR) principle.

The gene expression was measured using Fluorescence (Intercalating Dye or Probe) signals and numerical readings (1 = Positive if Fluorescence was present in the partition, and 0 = Negative if no signal was found. Fluorescence) The resulting value indicates the true amount of genetic material (Absolute Quantification) without the user needing to create a standard curve.

Advantages

  • Seamless integration and fast results Sample distribution into microwells inside the plate wells, thermal cycling, visualization, and data analysis - all combined in a single system. The use of dedicated nanoplates for digital PCR provides results in approximately 90 minutes, compared to more than 4 hours with other digital PCR systems. Standard plate formats automate the sample preparation process, further reducing hands-on time.
  • Scalable with high multiplexing capabilities QIAGEN QIAcuity system is available in various models with different bandwidths - 1, 4, and 8 tablets. The systems contain up to 5 detection channels, which allows for maximum multiplexing in sample analysis, thereby comparing QIAquity digital PCR technology with the capabilities of traditional real-time PCR and surpassing the capabilities of other digital PCR systems on the market.
  • Various Plate Configurations The QIAquity System offers various types of nanoplates with microwells to suit the different performance and assay sensitivity requirements. For example, for applications requiring higher throughput, such as gene expression analysis, dedicated 96-sample plates will provide a sample distribution of 8,000 microwells. For more sensitive applications such as the detection of rare mutations, QIAGEN offers plates that can analyze up to 24 samples with a sample distribution of 36,000 microwells.

Applications

The QIAGEN QIAcuity instruments, together with the Nanoplates and the PCR kits, enable digital PCR applications, including:
  • Rare mutation detection
  • Copy number variation analysis
  • Gene expression analysis
  • Pathogen detection
  • Genotyping
  • miRNA research

Software

The Software Suite provided with the instrument and installed on a separate computer controls one or multiple QIAcuity instruments, either connected directly to one instrument or using an existing local area network (LAN). Using the Software Suite, digital PCR experiments, samples, and reaction mixes can be defined, assigned to Nanoplates, and transferred to the QIAcuity instrument. After the run, data can be analyzed, reports can be created and data can be exported for external analysis.

The software provides multiple template functionalities to make repeating plate layouts or plate run parameters easily accessible, further transforming your digital PCR experience.

When integrated into a local area network, the computer is hosting the QIAcuity Software Suite function as a server that is accessible via LAN to other computers serving as clients. This enables multiple users to access the software from other rooms or offices and analyze data via a standard browser without the need to install the software on multiple computers or access and exchange data via internet connections.

Multiplex detection of oncogenic mutations using LNA-based assays on the QIAcuity digital PCR system.

Multiplex detection of oncogenic mutations using LNA-based assays on the Qiagen QIAcuity digital PCR system Digital PCR (dPCR) enables specific and sensitive detection of genetic alterations in oncogenes. It is particularly suited for the analysis of rare mutations in precious samples such as circulating cell-free DNA (ccfDNA) from blood and other body fluids. For reliable detection of single base exchanges at low frequency, both, a reproducibly working dPCR system and optimized PCR assays, are indispensable.

Locked Nucleic Acid (LNA)-enhanced oligonucleotides with substantially high levels of affinity for their complementary sequences make these assays excellent tools for the detection and discrimination of highly similar DNA targets. Here, we discuss simultaneous, high-sensitivity detection of up to three different mutations using QIAGEN QIAcuity dPCR system in combination with the dPCR LNA Mutation Assays.

Ordering Information 
Description
QIAcuity One, 2plex Device One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit.1 year warranty on labor, travel, and parts also included.
QIAcuity One, 2plex System One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
QIAcuity One, 2plex System FUL-2 One-plate digital PCR instrument for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
QIAcuity One, 5plex Device One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
QIAcuity One, 5plex System One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
QIAcuity One, 5plex System FUL-2 One-plate digital PCR instrument for detecting up to 5 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
QIAcuity Four Platform System Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit.1 year warranty on labor, travel, and parts.
QIAcuity Four Platform System FUL-2 Four-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.
QIAcuity Eight Platform System Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, and 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts.
QIAcuity Eight Platform System FUL-2
Eight-plate digital PCR instrument for detecting up to 5 fluorescent dyes, notebook computer, barcode scanner, nanoplate roller, USB flash memory and QIAcuity Software Suite: includes installation, training, Full Agreement for 2 years with a 2 business day response time, and 2 preventive maintenance visits.

Include:

  • QIAcuity EG PCR Kit (1 ml) 1 ml 3x concentrated QIAcuity EvaGreen Mastermix, 2 x 1.9 ml Water
  • QIAcuity EG PCR Kit (5 ml) 5 x 1 ml 3x concentrated QIAcuity EvaGreen Mastermix, 8 x 1.9 ml Water
  • QIAcuity EG PCR Kit (25 ml) 5 x 5 ml 3x concentrated QIAcuity EvaGreen Mastermix, 4 x 20 ml Water
  • Training on the QIAcuity
  • Starter package (1x pack of QIAcuity Nanoplate 26k 24-well and 1x QIAcuity master mix)
  • End-point cycler for routine applications (QIAamplifier 96)

Reference Link

QIAcuity One, 2plex System
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