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Cobas Liat PCR
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Cobas Liat PCR System is a rapid, point-of-care, RT-PCR test device that can be used to determine whether it is COVID-19 or the flu. 

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Cobas Liat

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Cobas Liat PCR System is a rapid, point-of-care, RT-PCR test device that can be used to diagnose influenza infection. The system consists of a small bench-top analyzer and a pencil-sized assay tube. It was originally developed by iQuum, and acquired by Roche in 2014. A nasopharyngeal (NP) swab collects a respiratory secretion sample from patients with suspected influenza.

The sample is then transferred by pipette into the single-use assay tube and loaded into the analyzer. It is compressed at different points and times, releasing reagents and moving the sample from one tube segment to another. The presence or absence of influenza A and influenza B is determined using an established nucleic acid assay that detects viral RNA, using RT-PCR. Results are reported in approximately 20 minutes.

Detection of Influenza A and B Viruses and Respiratory Syncytial Virus by Use of Clinical Laboratory Improvement

Cobas Liat was found to have the best performance and agreement compared to the standard RT-PCR for COVID-19 detection. With further research, these rapid tests can be employed in the large-scale screening of COVID-19.

"In rtRT-PCR, the method used by both the Roche cobas Liat and the Cepheid Xpert Xpress, the amplification of target DNA can be monitored using emitted fluorescence as the reaction progresses, generating a positive result once a fluorescent signal crosses a specified threshold."

Cobas Liat system SARS-CoV-2 & Influenza A/B nucleic acid test

524 samples were tested with this method. It is an automated multiplex real-time RT-PCR assay for in-vitro qualitative detection and differentiation of SARS-CoV-2, influenza A, and influenza B virus RNA in nasopharyngeal and nasal swabs.

The assay targets both the ORF1 a/b non-structural region and nucleocapsid protein gene that are unique to SARS-CoV-2. An Internal Process Control (IPC) is present to control for adequate processing of the target virus through steps of sample purification, nucleic acid amplification, and to monitor the presence of inhibitors in the RT-PCR processes.

The results are displayed as SARS-CoV-2 detected, not detected, or invalid within 20 minutes. It has got FDA emergency authorization for use.

Cobas Liat System Features:

  • The Cobas Liat System is a rapid, reverse transcription (RT)-polymerase chain reaction (PCR) point-of-care test that may be as effective as gold standard (lab-based) methods for diagnosing influenza A and influenza B.
  • Rapid RT-PCR tests for influenza may be better at detecting the presence or absence of influenza than other types of rapid influenza detection tests.
  • There is limited evidence comparing the Cobas Liat System with other rapid influenza detection tests.
  • Canadian pricing for the Cobas Liat System is not available.
  • There is limited evidence for using rapid RT-PCR tests outside a laboratory setting.

Cobas Liat System is a compact, fast, and easy-to-use real-time PCR system designed to test in Point of Care environments, such as primary care practices, pharmacies, and hospital emergency laboratories.

The system includes the  cobas® Liat analyzer, a small benchtop analyzer whose smooth operation is ensured by a wide range of internal instrument controls, and which has a  growing portfolio of assays, including cobas® Influenza kits. A / B, cobas® Influenza A / B, and RSV, cobas® Strep A and cobas® Clostridium difficile, constantly expanding into new assays for other infectious diseases.

Cobas Liat System Advantage

  • Small -  The analyzer is smaller than a shoebox and is conveniently designed to be placed in tight spaces.
  • Simple:  the cobas® Liat® system is fully automated and has a touch screen that guides the user through the various steps of the technique, requiring minimal training and very little user manipulation time.
  • Fast -  Show results in 20 minutes or less. This allows individual tests to be carried out, and urgent reports can be issued.
  • Safe -  Design of techniques in a closed reaction tube eliminates operator contact with reagents or other chemicals. In addition, the closed system with multiple controls reduces possible human errors.
  • Connected:  The cobas® Liat® system offers bi-directional connectivity to integrate all equipment in “Point of Care” environments with a central data management system.

The Cobas Liat PCR System delivers definitive results through easy and intuitive user handling, bringing you from sample preparation to results with no operator interpretation required. To meet diagnostic and patient care needs, Roche is committed to developing a wide range of assays for the Cobas Liat system.

This currently includes the following parameters:

Include:

  • Roche Diagnostics Cobas Liat Influenza A/B Assay
  • Roche Diagnostics Cobas Liat Strep A Control Kit
  • ROCHE DIAGNOSTICS CORPORATION COBAS LIAT SARSCOV2 KIT 20T/EA
  • ROCHE DIAGNOSTICS CORPORATION COBAS LIAT SARSCOV2 CTL 1KT/EA
  • Roche Diagnostics cobas™ Liat Cleaning Tool Kit
  • Power Cord for Cobas Liat PCR System Strep A Test Pack for Cobas Liat PCR System

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Cobas Liat
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