Illumina NextSeq 500 DNA Sequencer

The Illumina NextSeq 500 is a next-generation sequencing (NGS) system designed by Illumina, a leading company in the genomics field. The NextSeq 500 was developed to provide high-throughput sequencing capabilities in a benchtop format, making it suitable for a wide range of genomic applications.

The NextSeq 550 is a flexible and efficient platform that offers powerful high-throughput sequencing capabilities. Designed to finish each sequencing task independently and fast (within 30 hours), it is the newest high-throughput desktop sequencer to offer exome, transcriptome and whole-genome sequencing for all sizes of genomes. The flexibility of this platform allows a variety of flow cell configurations for optimized depth of coverage. Its sequencing capacity is up to 800 million paired-end reads and up to 400 million single reads per run.

Features and Capabilities:

• Sequencing Technology: The NextSeq 500 uses Illumina's proprietary sequencing-by-synthesis (SBS) technology. This method involves synthesizing the complementary strand of a DNA template and detecting the incorporated nucleotides in real-time.
• High Throughput: Despite its benchtop size, the NextSeq 500 offers high-throughput sequencing capabilities, allowing it to sequence whole human genomes, exomes, transcriptomes, and more.
• Flexible Output: The system provides flexibility in terms of output, accommodating both high-output and mid-output flow cells. This allows researchers to adjust the system based on the scale and needs of their experiments.
• Simplified Workflow: The NextSeq 500 has been designed with a streamlined workflow in mind. From sample preparation to sequencing and data analysis, the process is made efficient and user-friendly.
• Integrated Software: The system comes with onboard data analysis software, simplifying the bioinformatics process and offering primary data insights directly after sequencing.
• Applications: The NextSeq 500 is versatile and can be used for a wide range of applications including whole-genome sequencing, targeted sequencing, RNA sequencing, and more.

Instrument Dimensions:

• Size: Approximately 61 cm (W) x 63 cm (D) x 45 cm (H)
• Weight: Roughly 62 kg

Operating Conditions:

• Temperature: Typically 19°C to 25°C
• Humidity: Non-condensing relative humidity of 20% to 80%

Additional Features:

• Array Scanning: The NextSeq 550 uniquely integrates microarray scanning, allowing both high-throughput NGS and microarray scanning on a single platform.
• Software: Comes integrated with Illumina's sequencing control software and provides options for local or cloud-based data analysis.
• Applications: Suitable for a range of applications, including whole-genome sequencing, targeted sequencing, transcriptome sequencing, and more.

How It Works:

• Sample Preparation: The first step involves preparing the DNA or RNA samples, which includes library preparation and sample indexing.
• Cluster Generation: The prepared samples undergo a process called cluster generation, where individual DNA fragments are amplified on the surface of a flow cell to produce dense clusters of identical DNA fragments.
• Sequencing: Using Illumina's SBS technology, each DNA fragment in the clusters is sequenced. As nucleotides are incorporated into the growing DNA strand, a fluorescent signal is emitted, which corresponds to a specific nucleotide (A, T, C, or G). The system captures these signals in real-time.
• Data Analysis: After sequencing, the captured signals are translated into a sequence of nucleotides, resulting in raw sequence data. This data is then processed and analyzed to provide meaningful insights.

The NextSeq 550 System combines tried-and-true next-generation sequencing (NGS) and array capabilities with tunable outputs, enabling both small and large labs to scale to meet their needs. As a foundational instrument in the Illumina NGS system portfolio, the NextSeq 550 System is ideal for labs that want to expand beyond their current capacity and new labs interested in harnessing the complementary powers of sequencing and genotyping on a single instrument

The Illumina NextSeq 500 has become a popular choice for many laboratories due to its combination of high-throughput capabilities, flexibility, and benchtop convenience. It's a representation of how NGS technology has become more accessible and versatile, catering to both large-scale and smaller, targeted projects.

Summary

Leveraging proven SBS chemistry, user-friendly operation, and a streamlined DNA-to-results workflow, the NextSeq 550 System is an ideal lab partner. Access industry-leading NGS and array technologies on a single system, achieve the needed throughput and timing with multiple flow cell options, and see what can be accomplished with a NextSeq 550 System today.

unit was maintained under a contract by illumina from day one! 

machines software was Upgraded to RUO 4.0.1 SW ON 20-21 will provide also the old HDD'S

on 21/04/2023 the company decided to sell it 

dissembled from site professionally by illumina and certificated with a signed certification 

Reference

Manufacturer Information

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