Agilent BioTek Epoch 2 Microplate Spectrophotometer

BioTek Epoch 2 microplate spectrophotometer delivers excellent performance for UV-Vis absorbance measurements. Measurements can be performed in 6- to 384-well microplates, cuvettes, and microvolume samples with the available Take3 microvolume plate. The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays with higher wavelengths.

Epoch 2 is easy to use, and the optional touchscreen interface makes it simple to choose predefined protocols or to define custom programs. Endpoint, kinetic, spectral scanning, well area scanning modes, and incubation and shaking enable wide-ranging applications.

Epoch 2 includes monochromator-based individual wavelength selection or wavelength scanning from 200 to 999 nm, accommodating an extensive range of assays, including nucleic acid and protein quantification, ELISA, microbial growth, endotoxin, ROS assays, and enzyme kinetics.

The onboard software in the Epoch 2 eliminates the need for a separate computer and includes predefined standard protocols for rapid assay setup and recall. The optional color touchscreen interface easily accomplishes custom protocol definition, storage, and selection. Endpoint, kinetic, and spectral scanning applications are all available from the touchscreen. Data can be output to a USB flash drive or printer.

Agilent BioTek Epoch 2 Features:

• Spectrophotometer for single, dual, or multiple endpoints and kinetic reading measurements, as well as spectral and wafer surface scanning
• Large color touchscreen with WiFi, Bluetooth, and flash connectivity available (T models)
• Wavelength range: 200 to 999 nm, selectable in 1 nm steps
• For measurements in 2µl micro-volume samples, 6- to 384-well plates and standard cuvettes
• Full Gen5 data analysis software on the instrument or via Gen5 on an external PC
• Advanced shaking profiles, including linear, orbital, and double orbital
• Advanced incubator up to 65°C, with condensation control
• Compatible with Take3 plate for direct nucleic acid and protein assay of up to 16 2 µL samples simultaneously

How It Works

Filter-free UV-Vis wavelength selection from 200 – 999 nm

The monochromator-based optics in Epoch 2 allows the choice of wavelengths in 1 nm increments – no need to purchase filters! The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays.

Endpoint, kinetic and spectral scanning

The onboard software in Epoch 2 enables several read modes, including single and dual wavelength endpoint and kinetic measurements. Spectral scanning provides a convenient, quick spectrum of all defined samples. Under Gen5 software control, well-area scanning is also available, along with robust data analysis.

Compatible with 6- to 384-well plates and cuvettes for assay versatility

The optional cuvette port enables quick and easy 1 cm sample measurements, making Epoch 2 a versatile choice for assay workflows in various labware.

4-Zone™ incubation to 65 °C and Condensation Control

Some important applications like biofuels research and food & beverage safety and quality call for higher incubation temperatures than expected. Epoch 2 can meet these assay requirements. Kinetic assays are optimized with Condensation Control to prevent interference caused by condensation on plate lids.

Micro-volume (2 µL) measurements with the Take3 Plate

The Take3 Micro-Volume plates expand Epoch 2’s capabilities to measure 2 µL samples for direct DNA, RNA, and protein quantification. Get rapid results for up to 48 samples without additional instrumentation.

The available touchscreen interface and onboard software

Configurations:

Some Application Areas:

• ELISA
• Enzyme kinetics
• Nucleic acid and protein quantification
• Cell proliferation
• Cytotoxicity
• Spectral scanning
• Reactive oxygen species
• Food safety and quality
• Bacterial identification
• Total protein determination
• Nucleic acid purity assessment

Reference

• Manufacturer Information