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CLARIOstar Plus
$23,999.00
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Discover the cutting-edge BMG LabTech CLARIOstar Plus, a versatile microplate reader offering unparalleled sensitivity, flexibility, and speed for a wide range of high-throughput assays in drug discovery, genomics, and cell biology.

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CLARIOstar Plus

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CLARIOstar Plus is a modularly expandable high-performance microplate reader that meets all laboratories' current and future requirements. The device measures the leading detection technologies, including UV/vis absorbance, fluorescence intensity (incl. FRET), luminescence (incl. BRET), fluorescence polarization, time-resolved fluorescence (incl. TR-FRET), and AlphaScreen.

"I am using the clariostar plus plate reader for luminescence-, fluorescence-, FRET- and BRET-based assays. The plate readers shows a very good performance in all these modes and the user-friendly, intuitive control surface facilitates the fast setup of new experiments. The analysis software MARS enables a fast analysis and export of the raw data. What would improve the performance of the plate reader in dual-color assays (FRET & BRET), is the design of a filter cube that would allow for the simultaneous detection of both channels (as feasible with, e.g., the Neo2 plate from BioTek)."

CLARIOstar® multimodal reader, unlike readers equipped with classic monochromators, allows you to change the transmission slit width from 8 to 100 nm, significantly increasing its sensitivity. The presence of a library of fluorophores built into the software greatly simplifies the workflow.

Features

Advantages

  • It’s all on board: monochromator, filters, and spectrometer for maximum flexibility and sensitivity.
  • Simplified measurement setup: widest concentration and signal intensity range, auto-focus function
  • Optimized for long-term measurements with living cells: easy control of O2, CO2, de-, and re-oxygenation

Compared to its predecessor, the CLARIOstar Plus features Enhanced Dynamic Range and Auto Focus technology, which aim to eliminate the need for manual adjustment and make detection optimization easier than before. Specialized detectors (PMTs) for different measurement methods offer additional flexibility.

The CLARIOstar Plus combines the sensitivity of filters and the flexibility of monochromators in a revolutionary new technology: our patented LVF Monochromator.

Three technologies guarantee reliable results with every measuring method:

  • LVF monochromators combine flexibility and sensitivity for fluorescence intensity and luminescence measurements
  • Filters ensure excellent sensitivity for all measurement methods (excluding absorption)
  • The spectrometer records entire absorption spectra in a very short time

The ClarioStar Plus can perform modern cell/molecular/high throughput assays, including:

  • DNA,RNA and Protein quantification
  • Cell based assay / Long Term Kinetic Live Cell Assays
  • Enzyme and Kinetic assays
  • Genotyping  & Reporter gene assays
  • Molecular binding assays
  • ELSIA
  • Fluorescence Polarization
  • Bioluminescent Resonance Energy Transfer (BRET)
  • Time Resolved Fluorescence (TRF)

Sample Input:

  • Sample preparations in 6-384 well plates
  • The CLARIOstar Plus is equipped with Dual Reagent Dispensers (Dead volume 600ml)

Light Sources:

  • High energy xenon flash lamp
  • Dedicated Laser for Alphascreen / AlphaLISA / Alphaplex

Detection Modes:

  • UV/Vis absorbance
  • Fluorescence intensity
  • Luminescence
  • Fluorescence polarization
  • Time-resolved fluorescence
  • TR-FRET
  • Alphascreen / AlphaLISA / Alphaplex

Measurement Modes:

  • Top and bottom reading
  • Endpoint and Kinetic
  • Sequential multi-excitation
  • Sequential multi-emission
  • Spectral scanning (Absorbance, Luminescence, Fluorescence)
  • Ratiometric measurements
  • Well scanning

More time for your research – simple, automated, and flexible handling

The modularly expandable CLARIOstar Plus covers all the main detection technologies and is suitable even for highly demanding laboratory routines.

The monochromator lets you adjust the wavelengths and bandwidths for fluorescence and luminescence measurements quickly and easily.

Additional filters and an ultrafast UV/vis spectrometer for absorption measurements give you flexibility and speed.

The "Enhanced Dynamic Range" (EDR) feature simplifies the measurement setup and saves time.

Simply start your measurement, and the device will determine the ideal settings automatically. A single measurement covers the widest possible range of concentrations or signal intensities (8 orders of magnitude).

Perform long series of measurements with living cells feeling relaxed: thanks to the robust shaking and temperature control function, you can incubate your cells directly in the instrument. An atmospheric control unit (ACU) ensures the optimal composition of gases for each cell type. The ACU also allows de- and re-oxygenation, thus suitable for in vitro models of diseases such as ischemia/reperfusion.

Specification

Wavelength Range:
240 to 740 nm or 240 – 900 (red shifted PMT) for FI, FP, FRET, TRF, TR-FRET (filters),
240 to 740 nm for LUM (filters),
320 to 740 nm or 320 – 840 nm (red shifted PMT) for FI, FRET, TRF (monochromators),
240 to 740 nm for LUM (filters),
320 to 740 nm for LUM (monochromator),
220 to 1000 nm for ABS.
Optical System:
High energy xenon flash lamp,
Dedicated Laser for AlphaScreen® / AlphaLISA®,
Filters: Excitation and emission slides for 4 filters each,
LVF Monochromator™ : Excitation and Emission.
Detector(s): Low noise photomultiplier tube CCD spectrometer.
Light Source: High energy xenon flash lamp.
Measuring Time: Reading Times (Flying Mode): 9 seconds (96 wells), 14 seconds (384 wells), 27 seconds (1536 wells).
Plate Formats: 6 to 1536-well plates, LVis Plate with sixteen 2 µL microspots.
Detection System: DetectionFluorescence Intensity - including FRET, Fluorescence Polarization / Anisotropy, AlphaScreen® / AlphaLISA®, Luminescence (flash and glow) - including BRET, Time-Resolved Fluorescence - including TR-FRET, UV/Vis Absorbance Spectra

Include:

  • Installation and training BMG Labtech for CLARIOstar include installation and 1-day training for up to 4 users.
  • Bar code reader BMG Labtech for stacker III
  • Calibration BMG Labtech of EVPlate (681-101) with certification (recommended every two years)
  • EV Performance test plate BMG Labtech multimode validation plate. As listed under CLARIOstar
  • H3 Spoon BMG Labtech

Reference Library

CLARIOstar Plus

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